ABSTRAK

Diabetes melitus merupakan suatu penyakit metabolik yang ditandai dengan kenaikan kadar gula darah (hiperglikemia) yang melebihi nilai normal. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian senyawa Epigallocatechine gallate (EGCG) dalam menurunkan kadar glukosa darah dan gambaran histologi testis mencit induksi Alloxan monohydrate. Hewan coba yang digunakan sebanyak 24 ekor mencit jantan galur BALB/c yang dibagi menjadi 6 kelompok perlakuan, yaitu kelompok kontrol negatif (A), kontrol positif (B), Alloxan+glibenclamide (C), Alloxan+EGCG 7 mg/kgBB (D), Alloxan+EGCG 14 mg/kgBB (E) dan Alloxan+EGCG 21 mg/kgBB (F) masing-masing terdiri atas 4 ekor mencit. Induksi diabetes dilakukan menggunakan Alloxan monohydrate secara intraperitoneal dan pemberian EGCG dilakukan secara per oral pada mencit diabetik. Pengukuran kadar glukosa darah dilakukan pada hari ke- 0, 7, 14, dan 21 setelah pemberian perlakuan. Kadar gula darah puasa dianalisis menggunakan Anova dan menunjukkan nilai signifikansi 5% kemudian dilanjutkan dengan  uji Duncan yang menunjukkan bahwa ketiga kelompok dosis EGCG memiliki perbedaan aktivitas antidiabetes yang signifikan. Skoring histopatologi testis dan diameter tubulus seminiferus dianalisis menggunakan uji Kruskal wallis dan dilanjutkan dengan uji Mann-Whitney. Hasil penelitian menunjukkan rata-rata penurunan kadar glukosa darah pada kelompok A, B, C, D, E dan F berturut-turut sebesar -1,0; -30,25; 9,50; 30,00; 51,50; dan 53,25 mg/dL. Rata-rata skoring histopatologi spermatogenesis testis kelompok A, B, C, D, E dan F secara berturut adalah 7,0; 8,2; 8,6 dan  8,6 serta rata-rata diameter tubulus seminiferus pada kelompok A; B; C; D; E dan  F adalah 8,6; 45; 52; 60; 70 dan  82. Dari data tersebut dapat disimpulkan bahwa senyawa EGCG berpengaruh terhadap penurunan kadar gula darah dan gambaran histopatologi testis dan dosis yang dibutuhkan yaitu dosis 21 mg/kggBB yang menghasilkan penurunan sebesar 33,34%.

Kata Kunci: EGCG, alloxan, histopatologi spermatogenesis testis.

ABSTRACT

Diabetes mellitus is a metabolic disease characterized by an increase in blood sugar levels that exceed normal values. This study aimed to determine the effect of giving Epigallocatechine gallate (EGCG) compounds in reducing blood glucose levels and histological picture of testicular induction of Alloxan monohydrate mice. The experimental animals used were 24 male mice of the BALB / c strain which were divided into 6 treatment groups, namely the negative control group (A), positive control (B), Alloxan + glibenclamide (C), Alloxan + EGCG 7 mg / kgBB (D ), Alloxan + EGCG 14 mg / kgBB (E) and Alloxan + EGCG 21 mg / kgBB (F) each consisted of 4 mice. and Induction of diabetes was carried out using Alloxan monohydrate intraperitoneally and administration of EGCG was carried out orally in diabetic mice. Blood glucose level measurements were carried out on the 0th, 7th, 14th and 21st days after administration of the treatment. Fasting blood sugar levels were analyzed using Anova test and showed a significance value of 5% then continued with Duncan test which showed that the three groups of EGCG had significant differences in antidiabetic activity. Testicular histopathology scoring and seminiferous tubule diameter were analyzed using the Kruskal wallis test and continued with the Mann-Whitney test. The results showed an average decrease in blood glucose levels in groups A, B, C, D, E and F respectively by -1.0; -30.25; 9.50; 30.00; 51.50; and 53.25 mg / dL. The average histopathological scoring of testicular spermatogenesis of groups A, B, C, D, E and F in a row was 7.0; 8.2; 8.6 and 8.6 and the average diameter of seminiferous tubules in group A; B; C; D; E and F are 8.6; 45; 52; 60; 70 and 82. From these data it can be concluded that the EGCG compound has an effect on the decrease in blood sugar levels and testicular histopathology and the required dose was 21 mg / kggBB which results in a decrease of 33.34%.

Key Words: EGCG, alloxan, histopathology of testicular spermatogenesis