KARAKTERISTIK ANTIMIKROBA NANOFIBER PVA/GELATIN SEBAGAI PENUTUP LUKA

ANTIMICROBIAL CHARACTERISTICS OF PVA/GELATIN NANOFIBER AS WOUND DRESSING

Mikroorganisme yang terpapar ke permukaan menyebabkan perlu adanya pengembangan berupa agen antimikroba berbasis nanofiber. Nanofiber banyak diaplikasikan pada produk biomedis salah satunya sebagai penutup luka. Nanofiber yang dihasilkan dari larutan polyvinyl alcohol (PVA) dan gelatin dengan proses elektrospinning. Serat tersebut dilakukan variasi tegangan sebesar 17 kV, 20 kV, dan 23 kV. Dikarakterisasi dengan mikroskop optik, SEM, EDX, FTIR, UV-Vis, dan aktivitas antimikroba. Ukuran diameter nanofiber yang diukur dengan mikroskop optik menurun seiring bertambahnya tegangan. Spektroskopi UV-Vis menunjukkan nanofiber berbahan PVA/Gelatin menghasilkan nanofiber yang memiliki absorbansi tinggi seiring penambahan daya radiasi UV yang diberikan. Spektrum FTIR dengan rentang gelombang 400-4000 cm^-1 menunjukkan gugus fungsi yang terlihat yaitu, O-H stretching, C-H stretching, C-O stretching, C=O stretching dan C-H bending. Morfologi dan distribusi PVA/Gelatin ditunjukkan oleh SEM dengan diameter berkisar 140 – 160 nm, dengan unsur yang terdapat pada spektrum EDX yaitu C, O, dan N masing-masing 29,96%, 8,76%, dan 61,28%. Aktivitas antimikroba efektif hingga 47,5% dan efisien sampai dengan 2 jam. Sehingga nanofiber PVA/Gelatin dapat dijadikan acuan biomaterial sebagai penutup luka.

Microorganisms exposed to the surface cause the need for the development of nanofiber-based antimicrobial agents. Nanofiber is widely applied to biomedical products, one of which is as a wound dressing. Nanofiber produced from a solution of polyvinyl alcohol (PVA) and gelatin with an electrospinning process. The fiber is subjected to voltage variations of 17 kV, 20 kV, and 23 kV. Characterized by optical microscopy, SEM, EDX, FTIR, UV-Vis, and antimicrobial activity. The size of the nanofiber diameter as measured by optical microscopy decreased with increasing stress. UV-Vis spectroscopy showed that nanofibers made from PVA/Gelatin produced nanofibers with high absorbance as the UV radiation power was added. The FTIR spectrum with a wave range of 400-4000 cm-1 shows visible functional groups, namely, O-H stretching, C-H stretching, C-O stretching, C=O stretching and C-H bending. The morphology and distribution of PVA/Gelatin was shown by SEM with diameters ranging from 140 – 160 nm, with elements contained in the EDX spectrum namely C, O, and N respectively 29.96%, 8.76%, and 61.28%. Effective antimicrobial activity up to 47.5% and efficient up to 2 hours. So that PVA/Gelatin nanofibers can be used as a reference for biomaterials as wound dressings.