PENGARUH PENAMBAHAN Naphthalene Acetic Acid (NAA) DAN 6-Benzyl Amino Purine (BAP) TERHADAP PERTUMBUHAN PLANLET PISANG KEPOK KUNING (Musa Paradisiaca L.) PADA MEDIA Murashige and Skoog (MS) SECARA IN VITRO

EFFECT OF ADDING Naphthalene Acetic Acid (NAA) AND 6-Benzyl Amino Purine (BAP) ON THE GROWTH OF KEPOK KUNING BANANA (Musa Paradisiaca L.) PLANLET ON Murashige and Skoog (MS) MEDIA IN VITRO

Ketersediaan bibit Pisang Kepok Kuning (Musa paradisiaca L.) berkualitas semakin terbatas karena penyakit fisiologis seperti layu daun yang sering terjadi pada pohon pisang. Oleh karena itu, dilakukan teknik kultur jaringan sebagai solusi perbanyakan bibit Pisang Kepok Kuning karena bibit yang dihasilkan dalam jumlah banyak dan bebas mikroorganisme. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan NAA dan BAP terhadap pertumbuhan planlet Pisang Kepok Kuning (Musa paradisiaca L.) pada media MS secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap satu faktor perlakuan yaitu kombinasi konsentrasi NAA dan BAP dengan lima perlakuan; A (4 ppm NAA + 8 ppm BAP), B (5 ppm NAA + 7 ppm BAP), C (6 ppm NAA + 6 ppm BAP), D (7 ppm NAA + 5 ppm BAP), E (8 ppm NAA + 4 ppm BAP) serta satu kontrol: K (0 ppm NAA + 0 ppm BAP). Data pertumbuhan planlet meliputi jumlah tunas, jumlah daun, jumlah akar, dan tinggi planlet dianalisis menggunakan Uji ANOVA satu arah dilanjutkan Uji Duncan taraf 5%. Hasil penelitian menunjukkan penambahan NAA dan BAP berpengaruh terhadap pertumbuhan planlet Pisang Kepok Kuning pada media MS secara in vitro. Hal ini ditunjukkan pada parameter jumlah tunas dan jumlah daun optimal pada perlakuan A, Perlakuan B, dan Perlakuan C. Sedangkan jumlah akar dan tinggi planlet optimal pada perlakuan D, dan Perlakuan E.

The availability of quality Kepok Kuning Banana (Musa paradisiaca L.) seeds is increasingly limited due to physiological diseases such as leaf wilt that often occurs in banana trees. Therefore, tissue culture techniques were carried out as a solution for propagating Kepok Kuning Banana seeds because the seeds were produced in large quantities and free of microorganisms. This study aims to determine the effect of adding NAA and BAP on the growth of Kepok Kuning Banana (Musa paradisiaca L.) plantlets on MS media in vitro. This study used a completely randomized design with one treatment factor, namely a combination of NAA and BAP concentrations with five treatments; A (4 ppm NAA + 8 ppm BAP), B (5 ppm NAA + 7 ppm BAP), C (6 ppm NAA + 6 ppm BAP), D (7 ppm NAA + 5 ppm BAP), E (8 ppm NAA + 4 ppm BAP) and one control: K (0 ppm NAA + 0 ppm BAP). Plantlet growth data including number of shoots, number of leaves, number of roots, and plantlet height were analyzed using one-way ANOVA test followed by Duncan's test at 5% level. The results showed that the addition of NAA and BAP affected the growth of Kepok Kuning banana plantlets on MS media in vitro. This is shown in the optimal number of tuna and leaf number parameters in treatment A, treatment B, and treatment C. Meanwhile, the number of roots and plantlet height is optimal in treatment D, and treatment E.