Sintesis, Karakterisasi, dan Uji Aktivitas Antifungi Nanosilver dalam Sari Buah Mangrove (Sonneratia caseolaris) Khas Kota Surabaya

Synthesis, Characterization, and The Antifungal Activity Of Nanosilver In Mangrove Juice (Sonneratia caseolaris) Specially In Surabaya City

Perairan Indonesia memiliki potensi sumber daya laut yang tinggi, sehubungan dengan hal tersebut, Indonesia juga merupakan salah satu negara yang memiliki kawasan hutan mangrove (hutan bakau) terbesar di dunia. Salah satu potensi dan manfaat mangrove yang mulai banyak dikembangkan yakni buah mangrove sebagai sumber pangan, seperti sari buah mangrove pedada. Tujuan dari penelitian ini adalah untuk mensintesis, karakterisasi, dan uji aktivitas antifungi nanosilver dalam sari buah mangrove. Nanosilver disintesis menggunakan metode bottom up dengan hasil karakterisasi berupa panjang gelombang maksimum 415,20nm, absorbansi stabil selama empat minggu berturut-turut sebesar 1.9400; 1.9732; 1.9991; 1.9682, dan morfologi berbentuk bulat (spherical), dengan ukuran rata-rata 29,8761 nm. Pada penelitian ini sari buah mangrove dibagi menjadi tujuh perlakuan yakni K- tanpa penambahan agen antifungi (sebagai kontrol negatif), K+ dengan penambahan 0,1% natrium benzoat (sebagai kontrol positif), F1 hingga F5 dengan variasi kadar penambahan nanosilver sebesar 4%, 8%, 12%, 16%, dan 20%. Uji aktivitas antifungi dilakukan menggunakan metode Direct Microscopic Count (DMC) dengan teknik penghitungan langsung. Berdasarkan hasil penelitian ditunjukkan bahwa penambahan nanosilver memberikan pengaruh yang berbeda nyata di tiap konsentrasinya terhadap aktivitas antifungi dalam sari buah mangrove pedada. Perlakuan pada sampel F2 (dengan kadar penambahan nanosilver 8%) merupakan formulasi optimum dari semua perlakuan. 

The Indonesian waters have high potential for marine resources, therefore, Indonesia is also one of the countries that has the largest mangrove forest in the world. One of the potentials and benefits of mangroves that has begun to be widely developed is mangrove fruit as a food source, such as pedada mangrove juice. The purpose of this study was to synthesize, characterize, and test the antifungal activity of nanosilver in mangrove juice. Nanosilver was synthesized using the bottom up method with characterization results are maximum wavelength of 415.20 nm, stable absorption for four consecutive weeks of 1.9400; 1.9732; 1.9991; 1.9682, and its morphology is spherical, with an average size of 29.8761 nm. In this study, mangrove juice was divided into seven treatments namely K- without the addition of antifungal agents (as a negative control), K+ with the addition of 0.1% sodium benzoate (as a positive control), F1 to F5 with varying levels of nanosilver addition of 4%, 8%, 12%, 16% and 20%. Antifungal activity test was carried out using the Direct Microscopic Count (DMC) method with direct counting techniques. Based on the results of the study, it was shown that the addition of nanosilver had a significantly different effect at each concentration on the antifungal activity in pedada mangrove juice. Treatment on sample F2 (with the addition of 8% nanosilver content) is the optimum formulation of all treatments.