PENGARUH PEMBERIAN SIRUP PREBIOTIK UMBI YAKON TERHADAP AKTIVITAS ENZIM PENCERNAAN PADA DUODENUM Rattus norvegicus

THE EFFECT OF YAKON TUBER PREBIOTIC SYRUP ON DIGESTIVE ENZYME ACTIVITY IN DUODENUM Rattus norvegicus

Prebiotik adalah senyawa yang tidak dapat dicerna oleh enzim pencernaan dan dapat menstimulus pertumbuhan probiotik. Fruktooligosakarida merupakan prebiotik yang paling umum digunakan. Tujuan penelitian ini adalah mengetahui pengaruh pemberian sirup prebiotik umbi yakon terhadap aktivitas enzim amilase, protease, lipase pada duodenum Rattus norvegicus. Sampel penelitian adalah umbi yakon dari daerah Wonosobo dan hewan coba Rattus norvegicus. Hewan coba dibagi 4 kelompok. Kelompok  yang diberi pakan sumplemen air (P₀ = kontrol negatif), sirup prebiotik umbi yakon S₀ (P₁), sirup prebiotik umbi yakon S₁ (P₂), dan FOS komersial (P₃). Penentuan aktivitas enzim amilase, lipase, dan protease menggunakan instrumen spektrofotometer UV-Vis. Analisis statistik yang digunakan yaitu ANOVA dan PostHoc = LSD. Hasil penelitian menunjukkan bahwa pemberian sirup prebiotik umbi yakon S₁ dapat meningkatkan aktivitas enzim amilase, lipase dan protease secara signifikan (P < 0.05). Pada pemberian sirup prebiotik umbi yakon S₀ dapat meningkatkan aktivitas enzim amilase dan protease, sedangkan pada enzim lipase tidak ada pengaruh secara signifikan.

Prebiotics are compounds that cannot be digested by digestive enzymes and can stimulate the growth of probiotics. Fructooligosaccharides are the most commonly used prebiotics. The purpose of this study was to determine the effect of giving prebiotic syrup of yakon tuber to the activity of amylase, protease, and lipase enzymes in the duodenum of Rattus norvegicus. The samples of this research were yakon tubers from the Wonosobo area and the experimental animal Rattus norvegicus. Experimental animals were divided into 4 groups. The group that was fed with water supplements (P₀ = negative control), prebiotic syrup of yakon tuber S₀ (P₁), prebiotic syrup of yakon tuber S₁ (P₂), and commercial FOS (P₃). Determination of enzyme activity of amylase, lipase, and protease using UV-Vis spectrophotometer instrument. Statistical analysis used was ANOVA and Post Hoc = LSD. The results showed that the administration of prebiotic syrup of yakon tubers S₁ could significantly increase the activity of amylase, lipase and protease enzymes (P < 0.05). In the provision of prebiotic syrup of yacon tubers S₀ can increase the activity of amylase and protease enzymes, while the lipase enzyme has no significant effect.