Nanofiber PVA/ZnO Sebagai Material Antimikroba Pada Wound Dressings

PVA/ZnO Nanofibers As Antimicrobial Materials In Wound Dressings

Nanofiber sangat ideal untuk membentuk produk medis multifungsi, salah satunya wound dressings. Nanofiber wound dressings berbahan dasar polyvynyl alchol (PVA) dan zinc oxide (ZnO) menggunakan metode elektrospinning dengan variasi tegangan 15 kv, 16 kv, 18 kv, dan 20 kv, untuk mengetahui pengaruh tegangan terhadap morfologi, daya serap dan aktivitas antibakteri. Nanofiber yang dihasilkan memiliki permukaan halus dan diameter serat yang hampir seragam. Analisis SEM memperlihatkan diamater PVA 10% dengan perbesaran 50000X diperoleh 252 nm dan PVA/ZnO 20 kv dengan perbesaran 30000X diperoleh 231 nm, spektrum EDX terdapat unsur C, O, dan Zn masing-masing 37.40%, 62.14%, dan 0.46%. hasil analisa FTIR menunjukkan bahwa gugus fungsi yang terlihat pada sampel PVA/ZnO yaitu O-H, CH2, C-H, C-O, dan Zn-O. Transmisi UV-Vis menunjukkan bahwa PVA memiliki transmisi UV yang tinggi sebesar 12% tetapi semua nanofiber PVA/ZnO menunjukkan transmisi UV hampir 0%. Aktivitas antimikroba menunjukkan jumlah koloni bakteri yang dimiliki pada sampel PVA/ZnO lebih sedikit daripada kontrol. Rata-rata diameter nanofiber meningkat ketika nanopartikel ZnO ditambahkan, namun saat tegangan dinaikan maka diameter rata-rata nanofiber semakin kecil. Morfologi nanofiber sangat seragam dan tidak ada kristal nanopartikel ZnO, Jadi nanofiber PVA/ZnO dapat dipilh sebagai biomaterial yang ideal untuk wound dressings.

Nanofibers are ideal for forming multifunctional medical products, one of which is wound dressings. Nanofibers wound dressings made from polyvynyl alcohol (PVA) and zinc oxide (ZnO) using the electrospinning method with varying voltages of 15 kv, 16 kv, 18 kv, and 20 kv, to determine the effect of stress on morphology, absorption and antibacterial activity. The resulting nanofibers have a smooth surface and an almost uniform fiber diameter. SEM analysis showed that the diameter of PVA 10% with a magnification of 50000X was 252 nm and PVA/ZnO 20 kv with a magnification of 30000X was obtained 231 nm, the EDX spectrum contained elements of C, O, and Zn respectively 37.40%, 62.14%, and 0.46%. The results of the FTIR analysis showed that the functional groups seen in the PVA/ZnO samples were O-H, CH2, C-H, C-O, and Zn-O. UV-Vis transmission showed that PVA had a high UV transmission of 12% but all PVA/ZnO nanofibers showed a UV transmission of almost 0%. Antimicrobial activity showed that the number of bacterial colonies in PVA/ZnO samples was less than the control. The average nanofibers diameter increases when ZnO nanoparticles are added, but when tension is increased, the average diameter of the nanofibers decreases. The morphology of the nanofibers is very uniform and there are no ZnO nanoparticle crystals, so PVA/ZnO nanofibers can be chosen as an ideal biomaterial for wound dressings.