Penelitian ini bertujuan untuk mengetahui efek senyawa epigallocatechin 3-gallate (EGCG) dalam menurunkan kadar glukosa darah puasa (GDP), serta memperbaiki morfometri dan gambaran histopatologi hepar mencit jantan diinduksi aloksan. Penelitian dilakukan menggunakan 24 ekor mencit dikelompokkan dalam 6 kelompok: kelompok kontrol negatif, kontrol positif, EGCG dosis 7, 14, dan 21 mg/kgBB serta kelompok Glibenklamid 3 mg/kgBB. Pengukuran kadar GDP dilakukan pada hari ke-0, 7, 14, dan 21 menggunakan Glukometer. Pembuatan preparat histopatologi hepar menggunakan pewarnaan Hematoksilin-Eosin. Pengamatan morfometri meliputi berat, diameter dan permukaan hepar. Data kadar GDP, diameter dan berat hepar dianalisis menggunakan uji ANOVA, dilanjutkan uji Duncan. Data skor preparat hepar dianalisis dengan Kruskall-Wallis, dilanjutkan uji Mann-Whitney. Hasil penelitian menunjukkan terdapat penurunan kadar GDP dan skor kerusakan hepar pada setiap penambahan dosis EGCG. Data morfometri hepar menunjukkan bahwa pemberian EGCG mampu mengembalikan berat dan diameter hepar mendekati nilai normal. Hasil uji ANOVA menunjukkan terdapat efek pemberian EGCG terhadap kadar GDP dan berat hepar secara sangat signifikan (P<0,000). Dosis 21 mg/kgBB menghasilkan hasil terbaik dibandingkan dengan dosis EGCG lainnya, kontrol positif dan negatif, namun untuk diameter hepar pengaruhnya tidak signifikan (P<0,051). Hasil uji Kruskall-Wallis menunjukkan terdapat efek pemberian EGCG terhadap penurunan skor kerusakan hepar yang sangat signifikan (P<0,001). Dosis EGCG terbaik adalah 21 mg/kgBB. Hasil pengamatan morfometri hepar menunjukkan permukaan hepar tidak berbeda pada semua perlakuan. Dapat disimpulkan bahwa senyawa EGCG dalam berbagai dosis berpengaruh terhadap penurunan kadar GDP, morfometri dan histopatologi hepar mencit diabetes diinduksi aloksan. Dosis EGCG terbaik adalah 21 mg/kgBB.
Kata kunci: EGCG, aloksan, kadar GDP, morfometri hepar, histopatologi hepar
This study was aimed to know the effect of epigallocatechin 3-gallate (EGCG) compound in reducing the fasting blood glucose (FBG) level and repairing morphometry and liver histopathology of alooxan-induced male mice. The research was conducted using 24 mice grouped into 6 groups: negative control, positive control, EGCG group with doses of 7, 14, and 21 mg/kgBW and group of Glibenklamid with dose of 3 mg/kgBW. The measurement of FBG level was performed on day 0, 7, 14, and 21 using Glucometer. Preparations of histopathology liver section was done using Hematoksilin-Eosin staining. Observations of liver morphometry include weight, diameter and surface of the liver. FBG level, diameter and weight of liver was analyzed using ANOVA test followed by Duncan test. Data of liver histology score was analyzed usingKruskal-Wallis test followed by Mann-Whitney test. Result showed that there was a decrease in the level of FBG and liver damage score along with increase of dose of EGCG. Data of liver morphometry indicated that administration of EGCG can restored the weight and diameter of liver to approaching the normal value. ANOVA test result indicated that there was an effect of EGCG administrated on GDP and liver weight significantly(P<0.000). EGCG dose of 21 mg/kgBW produced the best result compared to the other dose of EGCG, positive and negative controls. Result of liver diameter was not significant (P<0,051). Kruskal-Wallis test results indicated that EGCG administration effected to decrease liver damage score significantly (P<0.001). The best dose of EGCG was 21 mg/kgBW. Liver morphometry observations showed that surface of liver was not different in all treatment. It can be concluded that the EGCG compound in various doses was effected FBG level, morphometry and histopathology liver in alloxan-induced diabetic mice. The best dose of EGCG was 21 mg/kgBW.
Keywords: EGCG, alloxan, FBG level, liver morphometry, liver histopathology