IDENTIFIKASI KANDUNGAN SENYAWA BIOAKTIF MIMI MINTUNA LOKAL INDONESIA (Carcinoscorpius rotundicauda) SEBAGAI KANDIDAT ANTIVIRAL COVID-19 INHIBITOR ACE2

IDENTIFICATION OF INDONESIAN LOCAL MIMI MINTUNA (Carcinoscorpius rotundicauda) BIOACTIVE COMPOUNDS AS CANDIDATES FOR ANTIVIRAL COVID-19 INHIBITOR ACE2

Carcinoscorpius rotundicauda adalah salah satu jenis Mimi Mintuna yang dapat ditemukan di Indonesia. Hewan ini merupakan hewan unik dengan banyak potensi terutama dalam bidang medis. Jenis Mimi Mintuna lain di luar negeri telah dikembangkan sebagai vaksin Covid-19. Tujuan penelitian ini adalah mengidentifikasi kandungan senyawa dalam dagingnya serta bioaktivitasnya sebagai antiviral Covid-19 inhibitor ACE2 melalui molecular docking. Tahapan penelitiannya meliputi identifikasi kandungan senyawa melalui GCMS, analisis molecular docking, dan analisis farmakokinetik ADME. Hasil analisis menunjukkan terdapat 17 senyawa dalam ekstrak metanol daging C. rotundicauda dengan tiga senyawa yang berpotensi sebagai inhibitor ACE2 (binding affinity) meliputi senyawa 2,5-dimethylphenol (-5,7 kkal/mol); 1,5-Bis-(trifluoroacetoxy)-pentane (-5,4 kkal/mol); dan 1-(carboxymethyl)pyridinium (-5,2 kkal/mol). Analisis farmakokinetik ADME menunjukkan ketiga senyawa memiliki profil ADME yang baik sebagai senyawa obat oral. Penelitian lebih lanjut seperti in vitro dan in vivo diperlukan untuk menguak kepastian bioaktivitasnya sebagai antiviral Covid-19.

Carcinoscorpius rotundicauda is a type of Mimi Mintuna found in Indonesia. This unique animal has a lot of potential, especially in the medical field. Other types of Mimi Mintuna abroad have been developed as a Covid-19 vaccine. This research aims to identify the compound content in the meat and its bioactivity as an antiviral Covid-19 ACE2 inhibitor through molecular docking. The stages of his research include the discovery of compound content through GCMS, molecular docking analysis, and ADME pharmacokinetic analysis. The results of the analysis showed that there were 17 compounds in the methanol extract of C. rotundicauda meat, with three compounds having the potential as ACE2 inhibitors, including 2,5-dimethylphenol (-5.7 kcal/mol); 1,5-Bis-(trifluoroacetoxy)-pentane (-5.4 kcal/mol); and 1-(carboxymethyl)pyridinium (-5.2 kcal/mol). ADME pharmacokinetic analysis showed that the third compound has a good ADME profile as an oral drug compound. Further research such as in vitro and in vivo is needed to reveal the certainty of its bioactivity as a Covid-19 antiviral.