Karakterisasi Nanofiber PVA-Ekstrak Daun Nangka Sebagai Wound Dressing

Characterization of PVA Nanofiber-Jackfruit Leaf Extract as Wound Dressing

Tanaman nangka berpotensi sebagai bahan pembuatan wound dressing karena mengandung senyawa flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri terutama bagian daun. Tujuan penelitian ini yaitu menganalisis pengaruh penambahan ekstrak daun nangka terhadap karakteristik nanofiber yang dihasilkan dan aktivitas antibakteri dari nanofiber PVA-ekstrak daun nangka terhadap bakteri Staphylococcus aureus dan Escherichia coli. Eskstraksi daun nangka dilakukan dengan metode maserasi yang kemudian difabrikasi dengan alat electrospinning hingga terbentuk nanofiber. Selanjutnya nanofiber dikarakterisasi diantaranya SEM-EDX, FTIR, porositas, antibakteri dan sifat mekanik melalui pengujian tarik. Hasil penelitian menjelaskan morfologi nanofiber PVA-Daun nangka 20% memiliki diameter fiber terkecil sebesar 144 nm. Hasil FTIR menunjukkan eksrak daun nangka berhasil tercampur dalam nanofiber PVA. Porositas terkecil pada PVA-Daun nangka 20% sebesar 39,93%. Kuat tarik sebear 27,25 Mpa dan nilai modulus Young 56,77 MPa serta elongasi 48,33%. Hal ini dapat mengurangi trauma yang disebabkan saat pengelupasan membran nanofiber pada kulit manusia. Jumlah koloni Nanofiber PVA-Daun nangka 20% lebih sedikit dibanding dengan kontrol tanpa nanofiber, sehingga mampu dan efektif dalam menghambat pertumbuhan bakteri S. aureus dengan efektivitas sebesar 87% dan E. coli sebesar 67%.

The jackfruit plant has the potential to be used as an ingredient for making wound dressings because it contains flavonoids, saponins and tannins which function as antibacterials, especially the leaves. The purpose of this study was to analyze the effect of adding jackfruit leaf extract on the characteristics of the resulting nanofiber and the antibacterial activity of PVA nanofiber-jackfruit leaf extract against Staphylococcus aureus and Escherichia coli bacteria. Extraction of jackfruit leaves was carried out by maceration method which was then fabricated using an electrospinning tool to form nanofibers. Furthermore, the nanofibers were characterized including SEM-EDX, FTIR, porosity, antibacterial and mechanical properties through tensile testing. The results of the study explained the morphology of 20% PVA-Jackfruit leaf nanofiber which had the smallest fiber diameter of 144 nm. The FTIR results showed that the jackfruit leaf extract was successfully mixed into the PVA nanofiber. The smallest porosity in PVA-20% jackfruit leaves was 39.93%. The tensile strength is 27.25 Mpa and the Young's modulus is 56.77 MPa and the elongation is 48.33%. This can reduce the trauma caused when exfoliating nanofiber membranes on human skin. The number of PVA-Jackfruit Leaf Nanofiber colonies was 20% less than the control without nanofiber, so it was capable and effective in inhibiting the growth of S. aureus bacteria with an effectiveness of 87% and E. coli of 67%.