Pengaruh Penambahan Filtrat Pisang Cavendish dan BAP (6-Benzyl Amino Purine) Pada Media Vacin and Went (VW) Terhadap Pertumbuhan Planlet Anggrek Cattleya Sp. Secara In Vitro

Effect of Cavendish Banana Filtrates and BAP (6-Benzyl Amino Purine) in Vacin and Went (VW) Media on the Growth of Cattleya Sp. Orchid Planlet In Vitro

Cattleya Sp. orchid production has decreased due to environmental changes and limitations in cultivation techniques, so a more effective propagation method is needed. Tissue culture with Vacin and Went (VW) media enriched with Cavendish banana filtrate and BAP can be an alternative to increase plantlet growth in vitro. This study aims to determine the effect and optimal concentration of adding Cavendish banana filtrate and BAP (6-Benzyl Amino Purine) to Vacin and Went (VW) media on the growth of Cattleya Sp. orchid plantlets in vitro. The research method used was an experimental method with a completely randomized design (CRD) using five treatments and five replications, namely control (K), P1 (10 g/L Cavendish banana filtrate + 2 ppm BAP), P2 (15 g/L Cavendish banana filtrate + 1.5 ppm BAP), P3 (20 g/L Cavendish banana filtrate + 1 ppm BAP), and P4 (25 g/L Cavendish banana filtrate + 0.5 ppm BAP). The parameters observed included the number of leaves, leaf color, and plantlet height. Data were analyzed using ANOVA and Duncan's further test with SPSS sofware. The research results show that treatments P1 (10 g/L Cavendish banana filtrate + 2 ppm BAP) and P2 (15 g/L Cavendish banana filtrate + 1.5 ppm BAP) had an optimal effect on the number of leaves, with averages of 27.00 and 28.60 leaves, respectively. The best leaf color, which is green yellow with a value of 5 and chroma of 8, was obtained with treatment P2 (15 g/L Cavendish banana filtrate + 1.5 ppm BAP). Treatments P3 (20 g/L Cavendish banana filtrate + 1 ppm BAP) and P4 (25 g/L Cavendish banana filtrate + 0.5 ppm BAP) had an optimal effect on the height of Cattleya orchid planlets, with an average height of 7.54 cm.