Pengaruh Ekstrak Daun Turi Merah (Sesbania grandiflora L.) terhadap Nilai Hematologi pada Mencit Jantan Diabetes

Effect of Red Turi Leaf Extract (Sesbania grandiflora L.) on Hematological Values in Diabetic Male Mice

Diabetes mellitus dapat mempengaruhi nilai hematologi darah, seperti kadar hemoglobin dan jumlah trombosit. Daun turi merah (Sesbania grandiflora L.) mengandung senyawa yang berpontensi untuk pengobatan diabetes dan perbaikan nilai hematologi. Tujuan penelitian ini ialah untuk mengetahui pengaruh ekstrak daun turi merah terhadap kadar hemoglobin dan jumlah trombosit pada mencit jantan diabetes, serta memperoleh dosis optimum. Penelitian dilakukan di Laboratorium Hewan Coba UNESA. Mencit diabetes dibuat dengan induksi aloksan 130 mg/kgBB. Penelitian menggunakan RAL dengan enam kelompok perlakuan yaitu kelompok negatif (KN), kelompok positif (KP), kelompok glibenclamide (KG), dan ekstrak (K1=250 mg/kgBB, K2=400 mg/kgBB, dan K3=500 mg/kgBB), masing-masing dengan pengulangan sebanyak empat kali serta perlakuan selama 14 hari. Kadar hemoglobin diukur menggunakan strip test hemoglobin dan jumlah trombosit menggunakan metode Brecher Cronkite. Data dianalisis menggunakan uji normalitas, uji homogenitas, One-Way Anova, uji Duncan, dan uji Tukey. Hasil analisis menunjukkan ekstrak daun turi merah berpengaruh terhadap kadar hemoglobin (p<0,05) dan jumlah trombosit (p<0,05). Berdasarkan hasil penelitian disimpulkan bahwa ekstrak daun turi berpengaruh terhadap kadar hemoglobin dan jumlah trombosit mencit diabetes. Dosis optimum dimiliki oleh dosis 400 mg/kgBB dengan kadar hemoglobin 19,32±1,48 dan jumlah trombosit 28,00±3,01 (×10⁴). 

Diabetes mellitus can affect blood hematological values, such as hemoglobin levels and platelet counts. Red turi leaves contain compounds that are potential to treat diabetes and improve hematological values. The purpose of this study was to determine the effect of red turi leaves extract on hemoglobin levels and platelet counts in diabetic male mice, and to obtain the optimum dose. The research was conducted at the Animal Experimental Laboratory of UNESA. Diabetic mice were made by 130 mg/kgBW alloxan induction. The study used RAL with six treatment groups i.e., negative group (KN), positive group (KP), glibenclamide group (KG), and extract (K1=250 mg/kgBW, K2=400 mg/kgBW, and K3=500 mg/kgBW), each with four repetitions. Extract treatment was conducted for 14 days. Hemoglobin levels were measured using hemoglobin test strips and platelet counts using the Brecher Cronkite method. The data gained were analyzed using normality test, homogeneity test, the One-Way ANOVA, Duncan’s, and Tukey test. The results of analysis showed that red turi leaves extract had an effect on hemoglobin levels (p<0.05) and platelet counts (p<0.05). Based on the results was concluded that turi leaf extract had an effect on hemoglobin levels and platelet counts indiabetic mice. The optimum dose was 400 mg/kgBW with a hemoglobin level of 19.32±1.48 and a platelet count of 28.00±3.01 (×10⁴).