KARAKTERISASI NANOFIBER PVA-MADU-KUNYIT SEBAGAI WOUND DRESSING ANTIBAKTERI

CHARACTERIZATION OF PVA-HONEY-TURMERIC NANOFIBER AS AN ANTIBACTERIAL WOUND DRESSING

Kunyit mempunyai potensi sebagai alternatif bahan dasar wound dressing karena mampu mengurangi intensitas trauma pada kulit. Kandungan senyawa madu dan kunyit berperan penting dalam menghambat pertumbuhan bakteri patogen seperti Staphylococcus aureus dan Escherichia coli. Penelitian ini bertujuan untuk mendeskripsikan karakteristik nanofiber PVA-Madu-Kunyit sebagai wound dressing antibakteri dan menganalisis efektivitas antibakteri nanofiber PVA-Madu-Kunyit sebagai wound dressing. Pembuatan nanofiber dilakukan dengan menggunakan metode elektrospinning kemudian dilakukan karakterisasi FTIR, SEM, uji antibakteri, dan uji kuat tarik. Hasil penelitian menjelaskan penambahan kunyit dan madu pada PVA menghasilkan nanofiber yang elongasi pada rentang sebesar 42%-67%. Namun, semakin banyak ekstrak kunyit ditambahkan mengakibatkan viskositas larutan PVA-Madu-Kunyit tinggi sehingga terjadi peningkatan ukuran nanofiber hingga 261 nm dan rata-rata ukuran pori berkisar 1 µm saat dilakukan uji SEM. Gugus fungsi dari penambahan bahan alam terdeteksi pada nanofiber PVA-Madu-Kunyit dibuktikan dengan munculnya peak gabungan baru dan terjadi pergeseran spektrum. Zona hambat terbentuk pada sampel nanofiber PVA-Madu-Kunyit membuktikan bahwa komposisi yang tepat dari banyaknya ekstrak kunyit memiliki pengaruh yang signifikan pada efektifitas antibakteri dengan munculnya luas zona bening 3,73 mm² pada bakteri S. aureus dan 0,61 mm² pada bakteri E. coli. Dari keseluruhan hasil karakterisasi yang telah dilakukan, nanofiber PVA-Madu-Kunyit yang memenuhi karakteristik dari wound dressing optimum pada variasi ekstrak kunyit sebesar 1,5 ml.

Kata kunci: nanofiber, kunyit, madu, wound dressing, antibakteri

Turmeric has potential as an alternative ingredient for wound dressings because it can reduce the intensity of trauma to the skin. Honey and turmeric compounds play an important role in inhibiting the growth of pathogenic bacteria such as Staphylococcus aureus and Escherichia coli. This study aims to describe the characteristics of PVA-Turmeric-Honey nanofiber as an antibacterial wound dressing and to analyze the antibacterial effectiveness of PVA-Turmeric-Honey nanofiber as a wound dressing. The manufacture of nanofibers was carried out using the electrospinning method and then carried out FTIR characterization, SEM, antibacterial test, and tensile strength test. The results of the study explained that the addition of turmeric and honey to PVA produced elongated nanofibers in the range of 42% -67%. However, the more turmeric extract added, the higher the viscosity of the PVA-Honey-Turmeric solution resulting in an increase in the nanofiber size up to 261 nm and an average pore size of around 1 µm during the SEM test. Functional groups from the addition of natural ingredients were detected in PVA-Honey-Turmeric nanofibers as evidenced by the emergence of new combined peaks and spectral shifts. The inhibition zone formed on the PVA-Honey-Turmeric nanofiber sample proved that the exact composition of the amount of turmeric extract used had a significant effect on antibacterial effectiveness with the appearance of a clear zone area of 3.73 mm² in S. aureus bacteria and 0.61 mm² in E. coli bacteria. From the overall results of the characterization that has been carried out, PVA-Honey-Turmeric nanofibers that meet the characteristics of the optimum wound dressing in the variation of turmeric extract are 1.5 ml.

Keywords: nanofiber, turmeric, honey, wound dressing, antibacterial