Pengaruh Variasi Konsentrasi Kloramfenikol terhadap Imprinting Factor pada Molecularly Imprinted Polymer
The Effect of Variations Concentration of Chloramphenicol on the Imprinting Factor of Molecularly Imprinted Polymer
Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi kloramfenikol terhadap nilai Imprinting Factor (IF) pada Molecularly Imprinted Polymer (MIP). Imprinting Factor adalah ukuran kekuatan interaksi polimer tercetak terhadap molekul template. Nilai IF dapat dihitung berdasarkan nilai kapasitas adsorpsi antara MIP dan polimer blanko (PB). MIP disintesis dari non-imprinted polymer (NIP) menggunakan metode presipitasi dengan kloramfenikol sebagai template, asam metakrilat (MAA) sebagai monomer, dan etilen glikol dimetakrilat (EGDMA) sebagai crosslinker. Hasil penelitian menunjukkan bahwa konsentrasi optimum adalah 10 ppm dengan nilai IF sebesar 5,005. Hasil adsorpsi isotermal kloramfenikol terbaik menggunakan MIP dapat dijelaskan dengan model Langmuir. nilai dari batas deteksi (LOD) sebesar 0,098 dan nilai dari batas kuantitasi (LOQ) sebesar 0,327.
Kata Kunci : kloramfenikol, MIP, adsorpsi, imprinting factor, isotermal
This research aimed is to determine the effect of chloramphenicol concentration on the imprinting factor (IF) value of the Molecular Imprinted Polymer (MIP). Imprinting Factor is a standard of the interaction power between printed polymer and template molecule. The IF value was calculated based on the adsorption capacity value between MIP and blank polymer (BP). MIP was synthesized from non-imprinted polymer (NIP) using the precipitation method with chloramphenicol as a template, methacrylic acid (MAA) as a monomer, and ethylene glycol dimethacrylate (EGDMA) as a crosslinker. The results showed that the optimum concentration was at 10 ppm with the IF value of 5,005. The isothermal adsorption result of Chloramphenicol using MIP can best be described by the Langmuir model. The limit of detection (LOD) value was 0.098 and the limit of quantification (LOQ) value was 0.327.
Keywords : chloramphenicol, MIP, adsorption, imprinting factor, isothermal