Telah dilakukan sintesis nanopartikel perak dengan metode reduksi kimia. Larutan induk ion perak direduksi dengan natrium sitrat dan penstabil PVP 3%. Karakterisasi menggunakan Spektrofotometer UV-Vis pada λ_maks 414 – 418,3 nm dihasilkan koloid perak berukuran nanometer yang relatif stabil selama masa penyimpanan 60 hari. Hasil uji TEM diperoleh bentuk klaster dominan adalah spherical dengan ukuran nanopartikel berkisar antara 18,60 – 42,97 nm. Nanosilver hasil sintesis diaplikasikan ke dalam variasi formulasi whitening cream F1 (10%), F2 (15%), dan F3 (20%) b/v dan uji aktivitas antibakteri terhadap Staphylococcus aureus menghasilkan rata-rata diameter daerah hambat masing-masing sebesar 7,36 ± 1,72 mm; 12,43 ± 2,43 mm; dan 19,26 ± 2,23 mm. Konsentrasi Hambat Minimum (KHM) adalah 10% yakni formulasi whitening cream dengan kandungan nanosilver terendah yang mampu menghambat pertumbuhan bakteri dengan kategori respon hambat sedang. Uji statistik (p ≤ 0,05) menunjukkan variasi penambahan nanosilver dalam whitening cream berpengaruh terhadap daya hambat pertumbuhan bakteri Staphylococcus aureus.

Kata kunci: nanosilver, PVP, aktivitas antibakteri, whitening cream.

The synthesis of silver nanoparticles has been carried out by chemical reduction method. The solution of silver ions is reduced with sodium citrate and 3% PVP as stabilizer. Characterization using UV-Vis spectrophotometer at λ_max 414 – 418,3 nm produced silver colloid in nanometer size which was relatively stable during the storage period of 60 days. TEM images show that the nanoparticles are dominant spherical in shape with ~ 18,60 – 42,97 nm dimensions. Synthesized nanosilver is applied to a variety of whitening cream formulations as an antibacterial. Antibacterial activity test on Staphylococcus aureus on formula F1 (10%), F2 (15%), and F3 (20%) w / v resulted in a mean local diameter of antibacterial inhibition of 7.36 ± 1.72 mm ; 12.43 ± 2.43 mm; and 19.26 ± 2.23 mm. The Minimum Inhibition Concentration (MIC) is 10%, namely the whitening cream formulation with the lowest nanosilver level that can help the growth of bacteria with a medium inhibitory response category. The statistical test (p ≤ 0,05) show that the variations in the addition of nanosilver in whitening cream affect the inhibitory growth of Staphylococcus aureus bacteria.

Keywords: nanosilver, PVP, antibacterial activity, whitening cream.