Pemberian Soya dalam Pengencer Air Kelapa-Kuning Telur Terhadap Kualitas Spermatozoa Sapi Simmental Suhu 4˚C

Provision of Soya in Coconut Water-Egg Yolk Diluent on Spermatozoa Quality of Simmental Bull at 4˚C 

    Efek penyimpanan suhu 4˚C mengakibatkan cold shock berdampak pada penurunan kualitas spermatozoa. Soya mengandung lestisin yang berfungsi sebagai krioprotektan ekstraseluler untuk melindungi membran spermatozoa. Tujuan penelitian untuk mengetahui pengaruh konsentrasi soya dalam pengencer dasar air kelapa 100 ml-10% kuning telur terhadap kualitas spermatozoa sapi Simmental. Penelitian menggunakan Rancangan Acak Lengkap terdiri atas 5 perlakuan: Pengencer dasar air kelapa 100 ml-10%kuning telur dengan konsentrasi soya 0g; 1,5g; 3g; 4,5g; dan 6g dengan 4 kali pengulangan. Data kualitas spermatozoa sapi Simmental meliputi motilitas, viabilitas dan integritas membran. Uji motilitas diamati dibawah mikroskop dengan perbesaran 400×. Uji viabilitas dilakukan menggunakan pewarna eosin-negrosin dan diamati dibawah mikroskop perbesaran 400×. Uji integritas membran dilakukan dengan larutan HOST dan diamati dibawah mikroskop perbesaran 400×. Data dianalisis menggunakan uji ANOVA satu arah dan dilanjutkan dengan uji Duncan untuk mengetahui perlakuan soya optimal. Hasil penelitian menunjukkan bahwa ada pengaruh penambahan soya terhadap kualitas spermatozoa semen sapi simmental sangat signifikan (P<0,00). Perlakuan 1,5 g soya menunjukkan hasil motilitas 55,78 ± 0,26%, viabilitas 64,92 ± 0,13%, dan integritas membran  53,97 ± 0,15% yang optimal dibandingkan dengan perlakuan lainnya. Disimpulkan bahwa penambahan soya 1,5 g soya merupakan perlakuan optimal dalam mempertahankan kualitas spermatozoa yang disimpan dalam suhu refrigerator 4˚C selama 3 hari.

    The effect of 4˚C temperature storage result in cold shock, resulting in a decrease in the quality of spermatozoa. Soya contains lesticin which functions as an extracellular cryoprotectant to protect the spermatozoa membrane. The purpose od the study was to determine the effect of soya concentration in the basic diluent of coconut water 100 ml-10% egg yolk on the quality of simmental bull spermatozoa. The study using a Complete Randomized Design consisted of 5 treatments: Coconut water base diluent 100 ml-10% egg yolk with a concentration of soya 0g; 1.5g; 3g; 4.5g; and 6g with 4 repetitions. Simmental bull spermatozoa quality data include motility, viability and membrane integrity. The motility test was observed with a 400× magnification under a microscope. The viability test was carries out using eosin-negrosin dye and observed with a 400× magnification under a microscope. The membrane integrity test was carried out using HOST fluid and observed with a 400× magnification under a microscope. The data were analyzed used a one-way ANOVA test and continued with the Duncan’s test to determine the optimal soya treatment. The result showed that there was an effect of adding soya on the quality of simmental bull semen spermatozoa very significantly (P<0.00).The treatment of 1.5 g of soya showed a motility result of of 55,78 ± 0,26%,  viability of  64,92 ± 0,13%, and membrane integrity of 53,97 ± 0,15% wich was optimal compared to other treatments. It was concluded that the addition of soya 1.5g of soya is the optimal treatments in maintaining the quality of spermatozoa stored in a refrigerator temperature of 4˚C for 3 day.